Listen In - Bitesize Bio Webinar Audios

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now!

RSS 0 0

Ep 57—Adding Multiplex Factor to Confocal Imaging with ZEISS LSM

Updated 3 months ago.

In this tutorial on confocal imaging, you will learn how you can:

- Capture weaker signals—and still get sound, reproducible data.
- Reach faster volumetric imaging without sacrificing resolution
- Increase data throughput for your imaging needs

Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes, cellular signaling, molecular trafficking, and diffusion events with real-time superresolution and high SNR.

The new Multiplex mode for Airyscan 2 uses smart illumination and detection schemes for parallel pixel acquisition on a confocal microscope. Scientists can now capture weaker signals, keep their context, and get statistically sound data.

Extending Airyscan imaging to larger model systems with lower expression levels, the new Multiplex mode increases acquisition speeds even further. You get superresolution and a 4 times higher SNR compared to traditional confocals. This novel concept allows rapid volumetric imaging with unprecedented resolution beyond what is available in traditional confocal systems today.

Airyscan 2 provides new data handling concepts, providing 6.6 times smaller data sizes and 5 times faster image reconstruction times. Further, optimized real time acquisition strategies employed with the LSM 9 family enable faster scan speeds for Airyscan 2, allowing higher data throughput.

For more information, Click Here —>