Mentors at Your Benchside

A good mentor can be the difference between success and failure. But what to do if you don't have help on hand? Mentors At Your Benchside provides curated help and advice from experienced researchers on various topics, from lab skills and techniques to career progression. Each short episode is bursting with easy-to-access help and advice that can improve your results and help you get the most out of your time in the lab.

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How Confluent Are Your Cells? A Beginner’s Guide

Updated about 1 month ago.

#21 — Do you know the difference between cell confluency and cell number? Can you measure cell confluency accurately? Cell confluency measurements are essential in cell-based experiments. Listen to this episode to learn what cell confluency is and the different quantification methods to measure it.

Read the original article to get visual guides to help you understand and measure your cell confluency accurately. [1]

Do you want more information to help you perfect your cell culture? Check out related articles to learn how to get the right passage number for your cells and how to culture adherent cells like HEK293s. [2,3]

1. How Confluent Are Your Cells? A Beginner’s Guide to Measuring Cell Culture Confluency. Available at:
2. What’s in a Number: Getting the Right Passage in Cell Culture. Available at:
3. What the HEK? A Beginner’s Guide to HEK293 Cells. Available at: